Journal: Scientific reports

Article Title: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

doi: 10.1038/srep43604

Figure Lengend Snippet: Figure 4. C/EBPβ is involved in miR-155-mediated attenuation of the stem cell self-renewal genes in NSCs. (A) Quantitative RT-PCR for C/EBP family members in NSCs overexpressing miR-155. The control was NSCs transfected with a scrambled sequence. The asterisk represents a significant difference (P < 0.05) compared with the control. (B) Western blots for C/EBPα and β after overexpression of miR-155. The control was NSCs transfected with a scrambled sequence. (C) Suppression of C/EBPβ expression, as well as levels of Msi1, Hes1 and Bmi1. NTC = no-treatment control. SCR = scrambled oligonucleotide sequence RNA-transfected control. Asterisks represent significant differences (P < 0.05) compared with the NTC and SCR groups. (D) Western blots for Msi1, Hes1 and Bmi1 for NSCs treated with C/EBPβ siRNA. (E) Binding motifs for C/ EBP transcription factors in the target regions used for ChIP-PCR analysis. (F) ChIP-PCR analysis for DNA immunoprecipitated by anti-C/EBPβ antibody.

Article Snippet: The chromatin was then immunoprecipitated at 4 °C overnight using 2 μ g anti-C/ebpβ antibody (sc-150x, Santa Cruz Biotechnology) and Normal mouse IgG antibodies (Santa Cruz Biotechnology).

Techniques: Quantitative RT-PCR, Control, Transfection, Sequencing, Western Blot, Over Expression, Expressing, Binding Assay, Immunoprecipitation

Journal: Scientific reports

Article Title: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

doi: 10.1038/srep43604

Figure Lengend Snippet: Figure 5. Suppression of target genes by miR-155 is conserved in human NSCs derived from human iPS cells. (A) NSCs induced from human iPS cells (a), and NESTIN and NF-M expressing neural cells derived from NSCs (b). (B) quantitative RT-PCR for C/EBPβ, MSI1, HES1 and BMI1 for NSCs derived from human iPS cells. The NSCs were treated with hsa-miR-155 mimic oligonucleotide (Mimic) or inhibitor to hsa-miR-155 (Inhibitor). The control was NSCs transfected with a scrambled sequence. Asterisks represent significant differences (P < 0.05) compared with the control. (C) Western blots for miR-155 targets for NSCs treated with the mimic oligonucleotide. (D) Western blots for miR-155 targets for NSCs treated with the inhibitor oligonucleotide.

Article Snippet: The chromatin was then immunoprecipitated at 4 °C overnight using 2 μ g anti-C/ebpβ antibody (sc-150x, Santa Cruz Biotechnology) and Normal mouse IgG antibodies (Santa Cruz Biotechnology).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Control, Transfection, Sequencing, Western Blot